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Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products


Citation

Tasrip, Nor Asmara and Khairil Mokhtar, Nur Fadhilah and Hanapi, Ummi Kalthum and Abdul Manaf, Yanty Noorzianna and Ali, Md. Eaqub and Cheah, Yoke Kqueen and Mustafa, Shuhaimi and Mohd Desa, Mohd Nasir (2019) Loop mediated isothermal amplification; a review on its application and strategy in animal species authentication of meat based food products. International Food Research Journal, 26 (1). pp. 1-10. ISSN 1985-4668; ESSN: 2231-7546

Abstract

Isothermal amplification is a technique that can amplify target DNA sequences at a single incubation temperature. Loop-mediated isothermal amplification (LAMP) is an extension of the isothermal DNA amplification technique that combines rapidity, simplicity, high specificity and sensitivity. Due to its overwhelming characteristics, LAMP has been explored for its feasibility in detecting various subjects, and recently in meat-based food products for DNA-based meat species authentication. It has been developed to target various meat species such as porcine, chicken, horse, and ostrich with sensitivity as low as 0.1 pg/μL. Further improvement with the use of magnetic beads, electrochemiluminescence and special dye such as calcein and crystal violet had increased the sensitivity of the LAMP assay. Other important characteristics were specific target gene primers as well as a shorter incubation time, warranting a good prospect for rapid testing authentication.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Halal Products Research Institute
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: LAMP; Meat; Meat speciation; Authentication; Adulteration
Depositing User: Nabilah Mustapa
Date Deposited: 08 Apr 2019 08:20
Last Modified: 08 Apr 2019 08:20
URI: http://psasir.upm.edu.my/id/eprint/67287
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