Effect of docosahexanoic acid (DHA) on quality of frozen-thawed bull semen using Tris extender

Citation

Kaka, Asmatullah and Haron, Abd Wahid and Yusoff, Rosnina and Degu, Nurhusien Yimer and Mada, Khumran Armiya'u and Behan, Atique Ahmed and Kaka, Ubedullah and Ebrahimi, Mahdi (2015) Effect of docosahexanoic acid (DHA) on quality of frozen-thawed bull semen using Tris extender. In: 6th Pan Commonwealth Veterinary Conference of the CVA and 27th Veterinary Association Malaysia Congress 2015 (PCVC6 & 27VAM), 23-27 Mar. 2015, The Royale Chulan Hotel, Kuala Lumpur, Malaysia. (pp. 191-193).

Abstract

This study was conducted to investigate the effect of DHA supplementation in Tris extender on frozen-thawed quality of bull sperms. Semen was collected by using an electro ejaculator. Ejaculates with acceptable quality were extended in Tris extender and divided into five groups to which 0 (control), 3, 5, 10, 15 ng/ml of DHA were added. The supplemented semen samples were sequentially incubated at 37°C for 15 minutes, cooled for 2 hours at 5°C, packaged into 0.25ml straws and cryopreserved in liquid nitrogen for 24 hours. Frozen-thawed semen evaluation resulted that significantly (P<0.05) higher values for all sperm parameters at 10ng/ml except DNA integrity which showed non-significantly (P>0.05) higher values at 10ng/ml. In conclusion, supplementation of Tris extender with DHA at 10ng/ml improves frozen-thawed quality of bull semen.

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Additional Metadata

Item Type:	Conference or Workshop Item (Paper)
Divisions:	Faculty of Veterinary Medicine
Keywords:	DHA; Bull semen; Cryopreservation
Depositing User:	Nabilah Mustapa
Date Deposited:	08 Mar 2019 06:24
Last Modified:	08 Mar 2019 06:24
URI:	http://psasir.upm.edu.my/id/eprint/67140
Statistic Details:	View Download Statistic

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