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Characterization of cucumber mosaic virus (CMV) causing mosaic symptom on Catharanthus roseus (L.) G. don in Malaysia


Mamat, Mazidah and Yusoff, Khatijah and Hashim, Habibuddin and Tan, Yee How and Lau, Wei Hong (2012) Characterization of cucumber mosaic virus (CMV) causing mosaic symptom on Catharanthus roseus (L.) G. don in Malaysia. Pertanika Journal of Tropical Agricultural Science, 35 (1). pp. 41-53. ISSN 1511-3701; ESSN: 2231-8542


A cucumber mosaic virus (CMV) isolate, causing leaf mosaic and distortion, malformed flowers or colourbreaking on the petals of Catharanthus roseus in Serdang, Selangor, Malaysia, was identified and designated as Malaysian periwinkle isolate (CMV-MP). The virus was spherical in shape with the size of 28.6 ± 0.48 nm in diameter with a central core. It was mechanically transmitted to various test plants which produced typical symptoms of CMV infection. The coat protein (CP) gene of the virus was amplified using reverse transcriptasepolymerase chain reaction (RT-PCR) and cloned in Escherichia coli using TOPO-TA vector. A single open reading frame of 657 nucleotides, potentially encoding for 218 amino acids was sequenced. A comparison with the CP genes of other CMV isolates indicated that CMV-MP shared 100% sequence homology to the CP gene sequence of C. roseus isolate of CMV in India. This is the first aetiology report on C. roseus in Malaysia showing natural mosaic disease symptoms supported with the nucleotide sequence analysis of the causal virus.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Publisher: Universiti Putra Malaysia Press
Keywords: Catharanthus roseus; Cucumber mosaic virus; Mosaic disease; Nucleotide sequence analysis; Coat protein gene
Depositing User: Muizzudin Kaspol
Date Deposited: 03 Nov 2015 04:02
Last Modified: 03 Nov 2015 04:02
URI: http://psasir.upm.edu.my/id/eprint/666
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