UPM Institutional Repository

Increased endothelial progenitor cells with age and grade of malignancy in astrocytic glioma patients


Das, Priscilla and Naing, Nyi Nyi and Wan Abdul Jamil, Wan Arfah Nadiah and Khin, Ohnmar Naing @ Noor Jan and Kueh, Yee Cheng and Rasalingam, Kantha (2018) Increased endothelial progenitor cells with age and grade of malignancy in astrocytic glioma patients. Pertanika Journal of Science & Technology, 26 (2). pp. 641-652. ISSN 0128-7680; ESSN: 2231-8526


Astrocytic gliomas are the most common primary brain tumours that originated from human glial cells. The tumours rely upon endothelial progenitor cells (EPCs) for neoangiogenesis. This study aimed to investigate the association between tissue resident EPCs in a brain tumour and normal adjacent tissue in relation to age and grade of astrocytic glioma. Astrocytic glioma patients (n=22), grade I to grade IV were consented from Hospital Universiti Sains Malaysia. Brain tumour tissue and normal adjacent brain tissue samples were obtained from each patient during surgery. The EPCs were stained with CD133+ and VEGFR-2+ markers. The tissue residents EPCs for each sample were determined using the immunofluorescence microscopy method. The age of the patients increased by disease severity in the following order (Grade I: 21.33±20.79 years) < (Grade II: 46.50±0.707 years) < (Grade III: 47.38±11.95 years) < (Grade IV: 48.44 ±10.66 years). The EPCs in brain tumour correlated significantly with the age of the patients with positive correlation (Spearman's rho correlation test, r=0.52; p=0.013). The tissue resident EPCs in the brain tumour (median=0.40, IqR=0.59) were significantly higher compared with the adjacent normal brain (median=0.067, IqR=0.29) (Wilcoxon Signed-Rank Test, Z stat=-3.587, p<0.001). Higher tissue resident EPCs were found in high grade (III & IV) glioma compared with EPCs in low grade (I & II) glioma (median=0.61, IqR=0.70 vs. median=0.26, IqR=0.30; z=-1.763 p=0.078). This study showed increased EPCs with age and grade of malignancy in astrocytic glioma patients. Therefore, targeting EPCs in gliomas based on tumour grade malignancy and age of the patients might be useful in effective treatment of astrocytic glioma.

Download File

11 JST Vol 26 (2) Apr 2018_JST-0787-2017_pg641-652.pdf

Download (765kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Publisher: Universiti Putra Malaysia Press
Keywords: Astrocytomas; Brain tumours; Endothelial progenitor cells; Glioma
Depositing User: Nabilah Mustapa
Date Deposited: 12 Feb 2019 07:02
Last Modified: 12 Feb 2019 07:02
URI: http://psasir.upm.edu.my/id/eprint/66293
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item