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Indirect immunofluorescence screening of potential arthritic autoantibodies in systemic lupus erythematosus


Selvaraja, Malarvili and Che Ku Daud, Che Ku Dahlan and Abdul Jalil, Marsitah and Md. Shah, Anim and Amin Nordin, Syafinaz and Ahmad Bajari, Zamzarina and Chong, Chou Min and Abdullah, Maha (2018) Indirect immunofluorescence screening of potential arthritic autoantibodies in systemic lupus erythematosus. Malaysian Journal of Medicine and Health Sciences, 14 (SP3). pp. 41-44. ISSN 1675-8544; ESSN: 2636-9346


Introduction: Joint involvement is common in systemic lupus erythematosus (SLE) patients, however, screening for joint specific autoantibodies in patients is not routinely performed. This may be due to the lack of known antigens and available tissue. The rat musculoskeletal tissue may be a suitable source of antigen to detect arthritic autoantibodies. Method: We tested plasma of SLE patients, with arthritis (N=9) and without arthritis (N=7) as well as plasma from normal individuals (N=7) on fresh sectioned tissue from rat plantar hind paw using indirect immunofluorescence method. Results: Binding of autoantibodies to striation in skeletal muscle cells in the tissue was clearly demonstrable in all samples from SLE with arthritis but not on slides incubated with plasma from normal or SLE without arthritis. Conclusion: Thus, rat plantar tissue may be suitable for detecting autoantibodies from SLE patients that may be involved in the pathogenesis of lupus arthritis.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Medicine and Health Science
Publisher: Faculty of Medicine and Health Sciences, Universiti Putra Malaysia
Notes: Special Issues: Updates in Clinical Lab and Experimental Medicine
Keywords: Autoantibodies; Lupus arthritis; Immunofluorescence
Depositing User: Nabilah Mustapa
Date Deposited: 11 Feb 2019 03:59
Last Modified: 11 Feb 2019 03:59
URI: http://psasir.upm.edu.my/id/eprint/66174
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