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Cyclodextrin glycosyltransferase biosynthesis by genetically engineered fermentative Lactococcus lactis in aerobic system


Citation

Amiri, Azin and Mohamad, Rosfarizan and Abdul Rahim, Raha and Saad, Wan Zuhainis and Ariff, Arbakariya (2015) Cyclodextrin glycosyltransferase biosynthesis by genetically engineered fermentative Lactococcus lactis in aerobic system. In: VI International Conference on Environmental, Industrial and Applied Microbiology (BioMicroWorld2015), 28-30 Oct. 2015, Barcelona, Spain. (p. 204).

Abstract / Synopsis

Lactococcus lactis is a facultative anaerobe famous for its fermentative metabolism. Its applications in different industries such as chemical, food and pharmaceuticals followed by its Generally Recognized as Safe status as well as being a model lactic acid bacterium and an interesting candidate for heterologous protein production/secretion have made this bacterium interesting to be investigated under different conditions. L. lactis has been traditionally employed in dairy and other food industries to produce fermented products. Therefore, research about L. lactis has been directed toward fermentation metabolism. Despite the classification of L. lactis as a facultative anaerobe, besides availability of extensive number of studies on its fermentative metabolism, some studies have pointed its ability to undergo respiratory growth in presence of a key chemical component in aerated cultures. It should be noted that L. lactis has been isolated from some plants. Intrinsically and in retrospect, it is not surprising if they endure and function under aerobic and respirative conditions. The key component for successful respiration of L. lactis is hemin. It is based on the fact that hemin cofactor is the only component being missed from the respiration electron transport chain in cell membrane of L. lactis. Therefore, L. lactis metabolic flux can be redirected to aerobic respiration by addition of hemin to the growth medium in presence of oxygen. It has been reported that aerobic respiration of L. lactis with the aid of hemin results in higher growth and biomass concentration, long-term survival and robustness. It is very applicable, as slow growth rate of L. lactis could be the single most important parameter making it less competitive protein expression system as compared to some industrially common strains such as Escherichia coli. Unfortunately, studies on L. lactis protein production under respiration conditions are very rare. While, there are various heterologous proteins being produced by recombinant L. lactis. Additionally, there is not enough information available about genetically engineered L. lactis behavior under aerobic respirative conditions, despite the fact that such interesting studies can lead to evolution in sciences and industries related to this strain.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
Institute of Tropical Forestry and Forest Products
Keywords: Recombinant Lactococcus lactis; Aerobic system; Respiration; Fermentation; Cyclodextrin glycosyltransferase
Depositing User: Nabilah Mustapa
Date Deposited: 02 Nov 2018 09:07
Last Modified: 02 Nov 2018 09:07
URI: http://psasir.upm.edu.my/id/eprint/65622
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