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Electrochemical immunosensor for detection of Mycobacterium tuberculosis


Mohd Azmi, Umi Zulaikha and Yusof, Nor Azah and Kusnin, Norzila and Md Noor, Siti Suraiya and Ong, Poh Shing and Ahmad Raston, Nurul Hanun (2018) Electrochemical immunosensor for detection of Mycobacterium tuberculosis. In: 2nd International Symposium on Advanced Materials and Nanotechnology (i-SAMN 2018), 15-16 Aug. 2018, The Everly Putrajaya, Malaysia. (pp. 54-55).


A rapid and sensitive sandwich electrochemical immunosensor was developed based on the fabrication of the graphene/polyaniline (GP/PANI) nanocomposite onto screen-printed gold electrode (SPGE) for detection of Mycobacterium tuberculosis (M. tuberculosis) antigen. The chemical bonding and morphology of GP/PANI-modified SPGE were studied by Raman spectroscopy and field enhance scanning electron microscopy-energy dispersive X-ray spectroscopy (FESEM-EDX), respectively. From both studies, it clearly showed that GP/PANI was successfully coated onto SPGE through drop cast technique. Cyclic voltammetry (CV) was used to study the electrochemical properties of the modified electrode. The effective surface area for GP/PANI-modified SPGE was enhanced when compared with bare SPGE. Differential pulse voltammetry (DPV) was used to detect the M. tuberculosis antigen. This proposed electrochemical immunosensor is sensitive, low sample volume, rapid, and disposable, which is suitable for tuberculosis detection in real samples.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Science
Institute of Advanced Technology
Keywords: Tuberculosis; Electrochemical sensors; Screen-printed gold electrode (SPGE); Graphene; Polyaniline
Depositing User: Nabilah Mustapa
Date Deposited: 08 Oct 2018 02:41
Last Modified: 08 Oct 2018 02:41
URI: http://psasir.upm.edu.my/id/eprint/65426
Statistic Details: View Download Statistic

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