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Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract


Citation

Alsultan, Qamar Mohammed Naji and Sijam, Kamaruzaman and Rashid, Tavga Sulaiman and Ahmad, Khairulmazmi and Awla, Hayman Kakakhan (2017) Investigation of phytochemical components and bioautography of Garcinia mangostana L. methanol leaf extract. Journal of Experimental Agriculture International, 15 (3). art. no. JEAI.31398. pp. 1-7. ISSN 2457-0591

Abstract / Synopsis

The aim of this work to identify chemical components of Garcinia mangostana leaves and test the antibacterial effect on Pseudomonas syringe pv. tomato and Xanthomonas oryzae pv. oryzae using bioautographic procedure. Phytochemical screening of methanolic extract showed that the leaf extract of G. mangostana rich in alkaloids, Flavonoids, Saponins, Tannins, Phenol, Terpenoids, Anthraquinone and Cardiac glycosides. A simple bioautographic procedure, involving spraying suspensions of the bacteria on thin layer chromatography (TLC) plates developed in solvents of varying polarities was used to detect the number of antibacterial compounds present in the extract. This activity was indicated by white spots against a red background on the TLC plates after spraying with 5% TTC aqueous solution. P. syringae and X. oryzae were inhibited by the most compounds separated on the TLC plates from the extract.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
DOI Number: https://doi.org/10.9734/JEAI/2017/31398
Publisher: SCIENCEDOMAIN International
Keywords: TLC; Antibacterial; Xanthomonas oryzae; Pseudomonas syringe; Phytochemical screening
Depositing User: Nabilah Mustapa
Date Deposited: 08 Oct 2018 10:25
Last Modified: 08 Oct 2018 10:25
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.9734/JEAI/2017/31398
URI: http://psasir.upm.edu.my/id/eprint/65352
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