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Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea


Niknejad, Azadeh and Abdul Kadir, Mihdzar and Kadzimin, Saleh and Abdullah, N. A. (2008) Induction of callus and protocorm-like bodies (PLBs) in Phalaenopsis gigantea. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 428-431).


An efficient and reproducible method for large-scale propagation of Phalaenopsis gigantea using leaf sections has been developed. Leaf sections from young plants were cultured on New Dogashima medium (NDM) supplemented with cytokinins (6-Benzylaminopurine (BAP), thidiazuron (TDZ), and kinetin (KIN), each at 0.01, 0.1, 0.5 and 1.0 mgL-1) alone and in combinations with (auxins anaphthaleneacetic acid (NAA), at 0.01, 0.1, 0.5 and 1.0 mgL-1). The explants developed calli and protocorm-like-bodies (PLBs) within 8 weeks of culture. Treatment TDZ in combination with auxins was found to be best for the induction of callus and PLBs.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Agriculture
Publisher: Sarawak Biodiversity Centre
Keywords: Phalaenopsis gigantea; Leaf sections; Callus; Protocorm-like-bodies (PLBs)
Depositing User: Nabilah Mustapa
Date Deposited: 04 Jul 2018 02:31
Last Modified: 04 Jul 2018 02:31
URI: http://psasir.upm.edu.my/id/eprint/64277
Statistic Details: View Download Statistic

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