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Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach


Citation

Shamsudin, Mariana Nor and Mohd Yaakob, Haslinda Ayu and M., Habsah (2008) Sustainable anti-MRSA Gracilaria changii extract through molecular biotechnology approach. In: Biodiversity and Biotechnology Symposium 2008, 19-21 Nov. 2008, Kuching, Sarawak, Malaysia. (pp. 297-299).

Abstract

Global bacterial infection management has a universal trend involving the fight against antibiotic resistant strains. The pressing microbial resistance problem incurred high infectious disease burden and cost constraints, requiring application of newer, more expensive agents. An approach for treatment against an antibiotic resistant pathogen, MRSA, involving a combination therapy is investigated. In the present study, synergy was determined by the checkerboard assay. The effect in combination of commercial antibiotic and seaweed extract were screened on MRSA strain. Following the disc diffusion test, the checkerboard assay, giving the FIC index of 0.93 indicate that the seaweed extract works in synergy with commercial antibiotics and it has the potential as a resistance modulator. In view of the fact that there was positive effect of the extract, further study were conducted molecularly on several genes to study the effect of the extract in combination with antibiotic toward the selected genes through RT-PCR analysis as compared to effect of the extract alone. The synergy effect on resistant genes of MRSA, namely mecA and mecI showed different nucleotide sequence changes as compared to untreated. The promising application of local G. changii as an antiMRSA agent is an added value to mega-diversity tropical flora.


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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
Publisher: Sarawak Biodiversity Centre
Keywords: Antimicrobial effects; Seaweed; MRSA strains; Antibiotic
Depositing User: Nabilah Mustapa
Date Deposited: 04 Jul 2018 02:31
Last Modified: 04 Jul 2018 02:31
URI: http://psasir.upm.edu.my/id/eprint/64273
Statistic Details: View Download Statistic

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