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Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium


Md. Zin, Noor Suffiah and Padrilah, Siti Nadzirah and Abdul Rahman, Mohd Fadhil and Sim, Han Koh and Khalid, Ariff and Abd. Shukor, Mohd. Yunus (2016) Isolation and characterization of a 2,4-dinitrophenol-degrading bacterium. Bioremediation Science and Technology Research, 4 (2). pp. 28-33. ISSN 2289-5892


2,4-dinitrophenol (2,4-DNP) is utilized in the production of wood preservatives, dyes, and also as a pesticide. Human acute (short-term) exposure to 2,4-DNP in humans by means of oral exposure are nausea or vomiting, sweating, headaches, dizziness, and weight reduction. Thus, the removal of this compound is highly sought. A 2,4-DNP-degrading bacterium (isolate 1) was isolated from a sample soil from Terengganu. This bacterium (isolate 1) was characterized as a rod Gram positive, non-sporulated, and non-motile bacterium. The bacterium is oxidase negative and had catalase positive activity and was able to grow aerobically on 2,4-dinitrophenol as the sole carbon source. This bacterium showed maximal growth on 2,4-DNP at the temperature optimum of 30 °C, pH 5.0 and was tolerant to 2,4-DNP concentration of up to 0.5 mM (0.092 g/L). This bacterium prefers to use urea as the nitrogen source in addition to yeast extract for mineral source and vitamin precursors.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Publisher: Hibiscus Publisher
Keywords: 2,4-dinitrophenol; 2,4-dinitrophenol-degrading bacterium; Bioremediation
Depositing User: Nabilah Mustapa
Date Deposited: 08 Jun 2018 00:51
Last Modified: 08 Jun 2018 00:51
URI: http://psasir.upm.edu.my/id/eprint/64024
Statistic Details: View Download Statistic

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