Citation
Chen, Yen-Ming and Chen, Li-Hua and Li, Meng-Pei and Li, Hsing-Fen and Higuchi, Akon and Kumar, S. Suresh and Ling, Qing-Dong and Alarfaj, Abdullah A. and Munusamy, Murugan A. and Chang, Yung and Benelli, Giovanni and Murugan, Kadarkarai and Umezawa, Akihiro
(2017)
Xeno-free culture of human pluripotent stem cells on oligopeptide-grafted hydrogels with various molecular designs.
Scientific Reports, 7.
art. no. 45146.
pp. 1-16.
ISSN 2045-2322
Abstract
Establishing cultures of human embryonic (ES) and induced pluripotent (iPS) stem cells in xeno-free conditions is essential for producing clinical-grade cells. Development of cell culture biomaterials for human ES and iPS cells is critical for this purpose. We designed several structures of oligopeptide-grafted poly (vinyl alcohol-co-itaconic acid) hydrogels with optimal elasticity, and prepared them in formations of single chain, single chain with joint segment, dual chain with joint segment, and branched-type chain. Oligopeptide sequences were selected from integrin- and glycosaminoglycan-binding domains of the extracellular matrix. The hydrogels grafted with vitronectin-derived oligopeptides having a joint segment or a dual chain, which has a storage modulus of 25 kPa, supported the long-term culture of human ES and iPS cells for over 10 passages. The dual chain and/or joint segment with cell adhesion molecules on the hydrogels facilitated the proliferation and pluripotency of human ES and iPS cells.
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