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Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta)


Citation

Lee, Wei Kang and Namasivayam, Parameswari and Abdullah, Janna Ong and Ho, Chai Ling (2017) Transcriptome profiling of sulfate deprivation responses in two agarophytes Gracilaria changii and Gracilaria salicornia (Rhodophyta). Scientific Reports, 7. art. no. 46563. pp. 1-14. ISSN 2045-2322

Abstract

Seaweeds survive in marine waters with high sulfate concentration compared to those living at freshwater habitats. The cell wall polymer of Gracilaria spp. which supplies more than 50% of the world agar is heavily sulfated. Since sulfation reduces the agar quality, it is interesting to investigate the effects of sulfate deprivation on the sulfate contents of seaweed and agar, as well as the metabolic pathways of these seaweeds. In this study, two agarophytes G. changii and G. salicornia were treated under sulfate deprivation for 5 days. The sulfate contents in the seaweed/agar were generally lower in sulfate-deprivated samples compared to those in the controls, but the differences were only statistically significant for seaweed sample of G. changii and agar sample of G. salicornia. RNA sequencing (RNA-Seq) of sulfate-deprivated and untreated seaweed samples revealed 1,292 and 3,439 differentially expressed genes (DEGs; ≥1.5-fold) in sulfate-deprivated G. changii and G. salicornia, respectively, compared to their respective controls. Among the annotated DEGs were genes involved in putative agar biosynthesis, sulfur metabolism, metabolism of sulfur-containing amino acids, carbon metabolism and oxidative stress. These findings shed light on the sulfate deprivation responses in agarophytes and help to identify candidate genes involved in agar biosynthesis.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1038/srep46563
Publisher: Nature Publishing Group
Keywords: Gracilaria changii; Gracilaria salicornia(Rhodophyta); Freshwater habitats; Agar
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 07 Nov 2018 08:17
Last Modified: 22 Oct 2019 01:35
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi= 10.1038/srep46563
URI: http://psasir.upm.edu.my/id/eprint/63598
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