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Toxic effects of copper on liver and cholinesterase of Clarias gariepinus


Padrilah, Siti Nadzirah and Ahmad, Siti Aqlima and Yasid, Nur Adeela and Sabullah, Mohd Khalizan and Daud, Hassan Mohd and Khalid, Ariff and Shukor, Mohd Yunus (2017) Toxic effects of copper on liver and cholinesterase of Clarias gariepinus. Environmental Science and Pollution Research, 24 (28). 225210 - 22523. ISSN 0944-1344; ESSN: 1614-7499


The release of pollutants, especially heavy metals, into the aquatic environment is known to have detrimental effects on such an environment and on living organisms including humans when those pollutants are allowed to enter the food chain. The aim of this study is to analyse the damage to Clarias gariepinus’ liver caused by exposure to different concentrations of copper. In the present study, samples of C. gariepinus were exposed to sub-lethal copper sulphate (CuSO4) concentrations (from 0.2 to 20.0 mg/L) for 96 h. Physiological and behavioural alterations were observed with respect to their swimming pattern, mucus secretion and skin colour. Mortality was also observed at high concentrations of copper. Histopathological alterations of the liver were analysed under light, transmission and scanning electron microscopies. The liver of the untreated group showed normal tissue structures, while histopathological abnormalities were observed in the treated fish under light and electron microscopes with increased copper concentrations. Histopathological abnormalities include necrosis, melanomacrophage, hepatic fibrosis and congested blood vessels. In addition, the enzyme activity of liver cholinesterase (ChE) was also found to be affected by copper sulphate, as 100% of cholinesterase activity was inhibited at 20.0 mg/L. Thus, liver enzyme activity and histopathological changes are proven to be alternative sources for biomarkers of metal toxicity.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1007/s11356-017-9923-3
Publisher: Springer Verlag
Keywords: Copper contamination; Cholinesterase; Clarias gariepinus; Biomarker; Acute toxicity; Histopathology effect
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 07 Nov 2018 08:16
Last Modified: 07 Nov 2018 08:16
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s11356-017-9923-3
URI: http://psasir.upm.edu.my/id/eprint/63593
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