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The antimalarial effect of curcumin is mediated by the inhibition of glycogen synthase kinase-3β


Citation

Ali, Amatul Hamizah and Sudi, Suhaini and Basir, Rusliza and Embi, Noor and Mohd Sidek, Hasidah (2017) The antimalarial effect of curcumin is mediated by the inhibition of glycogen synthase kinase-3β. Journal of Medicinal Food, 20 (2). 152 - 161. ISSN 1096-620X; ESSN: 1557-7600

Abstract / Synopsis

Curcumin, a bioactive compound in Curcuma longa, exhibits various pharmacological activities, including antimalarial effects. In silico docking simulation studies suggest that curcumin possesses glycogen synthase kinase-3β (GSK3β)-inhibitory properties. The involvement of GSK3 in the antimalarial effects in vivo is yet to be demonstrated. In this study, we aimed to evaluate whether the antimalarial effects of curcumin involve phosphorylation of host GSK3β. Intraperitoneal administration of curcumin into Plasmodium berghei NK65-infected mice resulted in dose-dependent chemosuppression of parasitemia development. At the highest dose tested (30 mg/kg body weight), both therapeutic and prophylactic administrations of curcumin resulted in suppression exceeding 50% and improved median survival time of infected mice compared to control. Western analysis revealed a 5.5-fold (therapeutic group) and 1.8-fold (prophylactic group) increase in phosphorylation of Ser 9 GSK3β and 1.6-fold (therapeutic group) and 1.7-fold (prophylactic group) increase in Ser 473 Akt in liver of curcumin-treated infected animals. Following P. berghei infection, levels of pro- and anti-inflammatory cytokines, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, interleukin (IL)-10, and IL-4 were elevated by 7.5-, 35.0-, 33.0-, and 2.2-fold, respectively. Curcumin treatment (therapeutic) caused a significant decrease (by 6.0- and 2.0-fold, respectively) in serum TNF-α and IFN-γ level, while IL-10 and IL-4 were elevated (by 1.4- and 1.8-fold). Findings from the present study demonstrate for the first time that the antimalarial action of curcumin involved inhibition of GSK3β.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1089/jmf.2016.3813
Publisher: Mary Ann Liebert
Keywords: Curcumin; In vivo; Inflammation; Malaria; Nuclear factor-kappa B (NF-κB)
Depositing User: Mas Norain Hashim
Date Deposited: 17 Jul 2018 16:40
Last Modified: 17 Jul 2018 16:40
Altmetrics: http:www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1089/jmf.2016.3813
URI: http://psasir.upm.edu.my/id/eprint/63402
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