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Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD)


Citation

Aziz, Nor Farahim and Halmi, Mohd Izuan Effendi and Wan Johari, Wan Lutfi (2017) Statistical optimization of hexavalent molybdenum reduction by Serratia sp. strain MIE2 using Central Composite Design (CCD). Journal of Biochemistry, Microbiology and Biotechnology, 5 (2). pp. 8-11. ISSN 2289-5779

Abstract / Synopsis

The conversion of hexavalent molybdenum (Mo (VI)) to Mo-blue is a bioremediation techniquewhich reduces the toxicity of molybdenum to a less toxic form by bacteria. The aim of this studyis to determine the optimum conditions of significant parameters or variables that affect thereduction of Mo (VI) to Mo-blue by the local isolate identified as Serratia sp. strain MIE2.Response Surface Methodology (RSM) was used in this study to optimize the reduction processusing Central Composite Design (CCD) as an optimization matrix. The optimum conditionspredicted by RSM using the desirability function for the reduction process were 20 mMmolybdate concentration, 3.95 mM phosphate, 6.25 pH and 25 g/L glucose and Mo-blueproduction occurred at the absorbance value of 20.5 at 865 nm. The validation of the predictedoptimum points showed the Mo-blue production occurred at the absorbance value of 21.85 witha deviation around 6.6 % from the RSM predicted value.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Environmental Studies
Publisher: Hibiscus Publisher
Keywords: Bioremediation; Molybdenum; Agriculture soil; Isolation; RSM
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 26 Sep 2018 09:54
Last Modified: 26 Sep 2018 09:54
URI: http://psasir.upm.edu.my/id/eprint/63285
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