UPM Institutional Repository

Isolation of Neospora caninum from a calf in Malaysia


Cheah, Tong Soon and Mattsson, Jens G. and Mohd Zain, Zaini and Abdullah Sani, Rehana and Jakubek, Eva Britt and Uggla, Arvid and Panchadcharam, Chandrawathani (2004) Isolation of Neospora caninum from a calf in Malaysia. Veterinary Parasitology, 126 (3). pp. 263-269. ISSN 0304-4017


In order to attempt isolate the protozoan parasite Neospora caninum, an N. caninum seropositive pregnant Sahiwal Friesian cross heifer from a large-scale dairy farm in Malaysia was kept for observation until parturition at the Veterinary Research Institute, Ipoh. The heifer gave birth to a female calf that was weak, underweight and unable to rise. Precolostral serum from the calf had an N. caninum indirect fluorescent antibody test titre of 1:3200. It died 12 h after birth and necropsy was performed. Brain homogenate from the calf was inoculated into 10 BALB/c mice that were kept for 3 months after which brain tissue from the mice was inoculated onto 24 h fresh monolayer Vero cell lines. The cell cultures were examined daily until growth of intracellular protozoa was observed. DNA of the organisms from the cell cultures was analyzed by PCR and DNA sequencing. DNA fragments of the expected size were amplified from the isolate using N. caninum-specific primers, and sequence analysis of ITS1 clearly identified the isolate as N. caninum. This is the first successful isolation of N. caninum from a bovine in Malaysia, and the isolate is designated Nc-MalB1.

Download File

PDF (Abstract)
Isolation of Neospora caninum from a calf in Malaysia.pdf

Download (84kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1016/j.vetpar.2004.08.012
Publisher: Elsevier
Keywords: Neospora caninum, Cattle-protozoa, Isolation, Malaysia
Depositing User: Azizan Arshad
Date Deposited: 13 May 2010 02:25
Last Modified: 27 Nov 2015 01:58
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.vetpar.2004.08.012
URI: http://psasir.upm.edu.my/id/eprint/6326
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item