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Removal of ammonium and nitrate in recirculating aquaculture systems by the epiphyte Stigeoclonium nanum immobilized in alginate beads


Mohamed Ramli, Norulhuda and Verdegem, M. C. J. and Md Yusoff, Fatimah and Zulkifely, M. K. and Verreth, J. A. J. (2017) Removal of ammonium and nitrate in recirculating aquaculture systems by the epiphyte Stigeoclonium nanum immobilized in alginate beads. Aquaculture Environment Interactions, 9. 213 - 222. ISSN 1869-215X; ESSN: 1869-7534


Incorporation of microalgae in recirculating aquaculture systems (RAS) would absorb the inorganic nitrogen and phosphorus, thus potentially contributing to water purification.Immobilization or entrapment of microalgal cells in spherical gels is a potential method to incor- porate microalgae in the RAS. Filamentous microalgae are presumed to suit the immobilization technique because the gels can serve as substrates for the microalgae to attach. In the first experiment of this study, growth and nitrogen uptake of Stigeoclonium nanum, a filamentous micro-alga, was compared when cultured using an immobilization technique or in a normal suspension.In the second experiment, immobilized S. nanum was cultured in 4 media with different total ammonia nitrogen (TAN) and nitrate-nitrogen (NO3-N) concentrations. The results showed a significantly higher algal growth and TAN removal by S.nanum immobilized in alginate than for S.nanum in free suspension culture. When both TAN and NO3-N were added to the culture medium, the uptake of TAN by immobilized S.nanum was significantly more efficient than NO3-N uptake.Our results indicated that S.nanum was able to grow immobilized in a medium, exhibiting a higher growth and TAN uptake than when the algae were in free suspension. S.nanum preferred ammonium over nitrate, which is suitable for RAS that require removal of the total ammonia which is produced by fish and by organic decomposition in the system.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture
Faculty of Engineering
Institute of Bioscience
DOI Number: https://doi.org/10.3354/aei00225
Publisher: Inter Research
Keywords: Alginate beads; Filamentous epiphyte; Immobilization; Nitrogen uptake; Stigeoclonium nanum; Aquaculture waste
Depositing User: Mas Norain Hashim
Date Deposited: 22 Oct 2018 08:49
Last Modified: 22 Oct 2018 08:49
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3354/aei00225
URI: http://psasir.upm.edu.my/id/eprint/62953
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