UPM Institutional Repository

Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia


Citation

Premarathne, Jayasekara M. K. J. K. and Anuar, Aimi Syazana and Thung, Tze Young and Satharasinghe, Dilan Amila and Jambari, Nuzul Noorahya and Abdul Mutalib, Noor Azira and Tang, John Yew Huat and Basri, Dayang Fredalina and Rukayadi, Yaya and Nakaguchi, Yoshitsugu and Nishibuchi, Mitsuaki and Radu, Son (2017) Prevalence and antibiotic resistance against tetracycline in Campylobacter jejuni and C. coli in cattle and beef meat from Selangor, Malaysia. Frontiers in Microbiology, 8. art. no. 2254. pp. 1-9. ISSN 1664-302X

Abstract

Campylobacter is a major foodborne pathogen frequently associated with human bacterial gastroenteritis in the world. This study was conducted to determine the prevalence and antibiotic resistance of Campylobacter spp. in the beef food system in Malaysia. A total of 340 samples consisting of cattle feces (n = 100), beef (n = 120) from wet markets and beef (n = 120) from hypermarkets were analyzed for Campylobacter spp. The overall prevalence of Campylobacter was 17.4%, consisting of 33% in cattle fecal samples, 14.2% in raw beef from wet market and 7.5% in raw beef from the hypermarket. The multiplex-polymerase chain reaction (PCR) identified 55% of the strains as C. jejuni, 26% as C. coli, and 19% as other Campylobacter spp. A high percentage of Campylobacter spp. were resistant to tetracycline (76.9%) and ampicillin (69.2%), whilst low resistance was exhibited to chloramphenicol (7.6%). The MAR Index of Campylobacter isolates from this study ranged from 0.09 to 0.73. The present study indicates the potential public health risk associated with the beef food system, hence stringent surveillance, regulatory measures, and appropriate interventions are required to minimize Campylobacter contamination and prudent antibiotic usage that can ensure consumer safety.


Download File

[img] Text
Prevalence and antibiotic resistance .pdf

Download (9kB)

Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Bioscience
Institute of Tropical Agriculture and Food Security
DOI Number: https://doi.org/10.3389/fmicb.2017.02254
Publisher: Frontiers Research Foundation
Keywords: Campylobacter; MPN-PCR; Antibiotic suspetibility; Beef; Prevalence
Depositing User: Mas Norain Hashim
Date Deposited: 21 Nov 2022 00:16
Last Modified: 21 Nov 2022 00:16
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3389/fmicb.2017.02254
URI: http://psasir.upm.edu.my/id/eprint/62838
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item