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Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells


Citation

Kumaran, Sekar and Pandurangan, Ashok Kumar and Shenbhagaraman, Ramalingam and Mohd Esa, Norhaizan (2017) Isolation and characterization of lectin from the Artist’s Conk medicinal mushroom, Ganoderma applanatum (Agaricomycetes) and evaluation of its antiproliferative activity in HT-29 colon cancer cells. International Journal of Medicinal Mushrooms, 19 (8). 675 - 684. ISSN 1521-9437; ESSN: 1940-4344

Abstract

he growth and lectin production of Ganoderma applanatum, a white rot fungus, was optimized in broth cultures. The fungus was found to have a higher growth rate and higher lectin activity when grown in a medium adjusted to pH 6.5 at 26°C under stationary conditions. Expression of lectin activity started in 5-day-old mycelial culture; maximum activity was expressed after the 15th day of incubation. Among the various carbon and nitrogen sources tested, the carbon source sucrose and the nitrogen source yeast extract support maximum growth and lectin production. Lectin from G. applanatum was purified by ammonium sulfate precipitation and ion exchange chromatography. The purified fraction revealed a single band with a molecular weight of 35.0 kDa. Moreover, carbohydrates such as mannitol, glucose, sucrose, maltose, mannose, galactose, sorbose, and fructose were found to inhibit the hemagglutinating activity of the lectin. The purified lectins from G. applanatum contain cytotoxic and proapoptotic activities against HT-29 colon adenocarcinoma cells.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1615/IntJMedMushrooms.2017021274
Publisher: Begell House
Keywords: Extracellular polysaccharides; Immunostimulation; Medicinal mushrooms; NK cell activity; Phagocytic assay; Splenocyte proliferation
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 21 May 2019 04:08
Last Modified: 21 May 2019 04:08
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1615/IntJMedMushrooms.2017021274
URI: http://psasir.upm.edu.my/id/eprint/62231
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