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Interaction between Pasteurella multocida B: 2 and its derivatives with bovine aortic endothelial cell (BAEC)


Citation

M. Kamal, Nuriqmaliza and Saad, M. Zamri and Masarudin, Mas Jaffri and Othman, Sarah (2017) Interaction between Pasteurella multocida B: 2 and its derivatives with bovine aortic endothelial cell (BAEC). BMC Veterinary Research, 13. art. no. 186. pp. 1-8. ISSN 1746-6148

Abstract

Background: Pasteurella multocida B:2 causes bovine haemorrhagic septicaemia (HS), leading to rapid fatalities in cattle and buffaloes. An attenuated derivative of P. multocida B:2 GDH7, was previously constructed through mutation of the gdhA gene and proved to be an effective live attenuated vaccine for HS. Currently, only two potential live attenuated vaccine candidates for HS are being reported; P. multocida B:2 GDH7 and P. multocida B:2 JRMT12. This study primarily aims to investigate the potential of P. multocida B:2 GDH7 strain as a delivery vehicle for DNA vaccine for future multivalent applications. Results: An investigation on the adherence, invasion and intracellular survival of bacterial strains within the bovine aortic endothelial cell line (BAEC) were carried out. The potential vaccine strain, P. multocida B:2 GDH7, was significantly better (p ≤ 0.05) at adhering to and invading BAEC compared to its parent strain and to P. multocida B:2 JRMT12 and survived intracellularly 7 h post treatment, with a steady decline over time. A dual reporter plasmid, pSRGM, which enabled tracking of bacterial movement from the extracellular environment into the intracellular compartment of the mammalian cells, was subsequently transformed into P. multocida B:2 GDH7. Intracellular trafficking of the vaccine strain, P. multocida B:2 GDH7 was subsequently visualized by tracking the reporter proteins via confocal laser scanning microscopy (CLSM). Conclusions: The ability of P. multocida B:2 GDH7 to model bactofection represents a possibility for this vaccine strain to be used as a delivery vehicle for DNA vaccine for future multivalent protection in cattle and buffaloes.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1186/s12917-017-1109-1
Publisher: BioMed Central
Keywords: Pasteurella multocida; Haemorrhagic septicaemia; Bovine aortic endothelial cell; Bactofection; DNA vaccine
Depositing User: Ms. Ainur Aqidah Hamzah
Date Deposited: 20 May 2022 07:54
Last Modified: 20 May 2022 07:54
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/s12917-017-1109-1
URI: http://psasir.upm.edu.my/id/eprint/62197
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