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In vivo carbon tetrachloride-induced hepatoprotective and in vitro cytotoxic activities of Garcinia hombroniana (seashore mangosteen)


Citation

Jamila, Nargis and Khan, Naeem and Khan, Amir Atlas and Khan, Imran and Khan, Sadiq Noor and Zakaria, Zainal Amiruddin and Khairuddean, Melati and Osman, Hasnah and Kim, Kyong Su (2017) In vivo carbon tetrachloride-induced hepatoprotective and in vitro cytotoxic activities of Garcinia hombroniana (seashore mangosteen). African Journal of Traditional Complementary and Alternative Medicines, 14 (2). 374 - 382. ISSN 0189-6016

Abstract

Background: Garcinia hombroniana, known as "manggis hutan" (jungle mangosteen) in Malaysia, is distributed in tropical Asia, Borneo, Thailand, Andaman, Nicobar Islands, Vietnam and India. In Malaysia, its ripened crimson sour fruit rind is used as a seasoning agent in curries and culinary dishes. Its roots and leaves decoction is used against skin infections and after child birth. This study aimed to evaluate in vivo hepatoprotective and in vitro cytotoxic activities of 20% methanolic ethyl acetate (MEA) G. hombroniana bark extract. Materials and methods: In hepatoprotective activity, liver damage was induced by treating rats with 1.0 mL carbon tetrachloride (CCl4)/kg and MEA extract was administered at a dose of 50, 250 and 500 mg/kg 24 h before intoxication with CCl4. Cytotoxicity study was performed on MCF-7 (human breast cancer), DBTRG (human glioblastoma), PC-3 (human prostate cancer) and U2OS (human osteosarcoma) cell lines. 1H, 13C-NMR (nuclear magnetic resonance), and IR (infrared) spectral analyses were also conducted for MEA extract. Results: In hepatoprotective activity evaluation, MEA extract at a higher dose level of 500 mg/kg showed significant (p<0.05) potency. In cytotoxicity study, MEA extract was more toxic towards MCF-7 and DBTRG cell lines causing 78.7% and 64.3% cell death, respectively. MEA extract in 1H, 13C-NMR, and IR spectra exhibited bands, signals and J (coupling constant) values representing aromatic/phenolic constituents. Conclusions: From the results, it could be concluded that MEA extract has potency to inhibit hepatotoxicity and MCF-7 and DBTRG cancer cell lines which might be due to the phenolic compounds depicted from NMR and IR spectra.


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Official URL or Download Paper: https://www.ncbi.nlm.nih.gov/pubmed/28573253

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.21010/ajtcam.v14i2.38
Publisher: Faculty of Pharmacy, D R P U
Keywords: Carbon tetrachloride; Garcinia hombroniana; Hepatoprotective activity; Cytotoxicity; NMR
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 16 Apr 2019 03:27
Last Modified: 16 Apr 2019 03:27
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.21010/ajtcam.v14i2.38
URI: http://psasir.upm.edu.my/id/eprint/62140
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