UPM Institutional Repository

Green-synthesized CdS nano-pesticides: toxicity on young instars of malaria vectors and impact on enzymatic activities of the non-target mud crab Scylla serrata


Sujitha, Vasu and Murugan, Kadarkarai and Dinesh, Devakumar and Pandiyan, Amuthvalli and Aruliah, Rajasekar and Hwang, Jiang Shiou and Kalimuthu, Kandasamy and Panneerselvam, Chellasamy and Higuchi, Akon and Aziz, Al Thabiani and Kumar, Suresh and A. Alarfaj, Abdullah and Vaseeharan, Baskaralingam and Canale, Angelo and Benelli, Giovanni (2017) Green-synthesized CdS nano-pesticides: toxicity on young instars of malaria vectors and impact on enzymatic activities of the non-target mud crab Scylla serrata. Aquatic Toxicology, 188. 100 - 108. ISSN 0166-445X; ESSN: 1879-1514


Currently, nano-formulated mosquito larvicides have been widely proposed to control young instars of malaria vector populations. However, the fate of nanoparticles in the aquatic environment is scarcely known, with special reference to the impact of nanoparticles on enzymatic activity of non-target aquatic invertebrates. In this study, we synthesized CdS nanoparticles using a green protocol relying on the cheap extract of Valoniopsis pachynema algae. CdS nanoparticles showed high toxicity on young instars of the malaria vectors Anopheles stephensiand A. sundaicus. The antimalarial activity of the nano-synthesized product against chloroquine-resistant (CQ-r) Plasmodium falciparumparasites was investigated. From a non-target perspective, we focused on the impact of this novel nano-pesticide on antioxidant enzymes acetylcholinesterase (AChE) and glutathione S-transferase (GST) activities of the mud crab Scylla serrata. The characterization of nanomaterials was carried out by UV–vis and FTIR spectroscopy, as well as SEM and XRD analyses. In mosquitocidal assays, LC50 of V. pachynema-synthesized CdS nanoparticles on A. stephensi ranged from 16.856 (larva I), to 30.301 μg/ml (pupa), while for An. sundaicus they ranged from 13.584 to 22.496 μg/ml. The antiplasmodial activity of V. pachynema extract and CdS nanoparticles was evaluated against CQ-r and CQ-sensitive (CQ-s) strains of Plasmodium falciparum. IC50 of V. pachynema extract was 58.1 μg/ml (CQ-s) and 71.46 μg/ml (CQ-r), while nano-CdS IC50 was 76.14 μg/ml (CQ-s) and 89.21 μg/ml (CQ-r). In enzymatic assays, S. serrata crabs were exposed to sub-lethal concentrations, i.e. 4, 6 and 8 μg/ml of CdS nanoparticles, assessing changes in GST and AChE activity after 16 days. We observed significantly higher activity of GST, if compared to the control, during the whole experiment period. In addition, a single treatment with CdS nanoparticles led to a significant decrease in AChE activity over time. The toxicity of CdS nanoparticles and Cd ions in aqueous solution was also assessed in mud crabs, showing higher toxicity of aqueous Cd ions if compared to nano-CdS. Overall, our results underlined the efficacy of green-synthesized CdS nanoparticles in malaria vector control, outlining also significant impacts on the enzymatic activity of non-target aquatic organisms, with special reference to mud crabs.

Download File

Green-synthesized CdS nano-pesticides.pdf

Download (80kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.aquatox.2017.04.015
Publisher: Elsevier
Keywords: Acetylcholinesterase; Anopheles stephensi; Biosafety; Glutathione S-transferase; Malaria vector; Nanosynthesis
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 12 Mar 2019 08:40
Last Modified: 12 Mar 2019 08:40
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.aquatox.2017.04.015
URI: http://psasir.upm.edu.my/id/eprint/61984
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item