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Pulmonary lesions associated with intratracheal Benzo(a)pyrene instillation in Sprague Dawley rats


Mustapha, Mazlina and Karim, Abdulkarim Jafar and Saad, Mohd Zamri and Mohamed Mustapha, Noordin (2011) Pulmonary lesions associated with intratracheal Benzo(a)pyrene instillation in Sprague Dawley rats. Pertanika Journal of Tropical Agricultural Science, 34 (2). pp. 367-372. ISSN 1511-3701; ESSN: 2231-8542


This study was conducted to assess acute exposure to very low dose of intratracheally instilled Benzo(a)pyrene (BaP) on the lungs of rats. A total of 30 rats were utilized in this study and they were randomly divided into 6 groups. The control group (G1) did not receive any treatment, whereas the rats in the remaining 5 groups were administered with 13.8 ng of BaP, which were then sacrificed at 1 hour (G2), 8 hours (G3), 16 hours (G4), 32 hours (G5), and 72 hours (G6) of post-instillation (p.i.). Morphological appearances of all the lungs of all the treated rats consisted of various degrees of congestion, mostly evident in G3 and early development of emphysema, as seen in G4. These worsened as time progressed as observed in G6. On the other hand, the histological findings of the lungs of the treated rats revealed that the lungs had underwent some changes that were characterized by progressive alveolar congestion, epithelialisation with emphysema and accompanied by infiltration of inflammatory cells predominantly with alveolar macrophages and some neutrophils. However, even with such lesions seen, there was no apparent manifestation of impairment of the pulmonary system.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Universiti Putra Malaysia Press
Keywords: BaP; Histological; Intratracheally; Lung; Morphological
Depositing User: Muizzudin Kaspol
Date Deposited: 03 Nov 2015 03:22
Last Modified: 22 Nov 2016 08:04
URI: http://psasir.upm.edu.my/id/eprint/6183
Statistic Details: View Download Statistic

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