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Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea


Kamarudin, Amirah Nor and Lai, Kok Song and Lamasudin, Dhilia Udie and Abu Seman, Idris and Balia Yusof, Zetty Norhana (2017) Enhancement of thiamine biosynthesis in oil palm seedlings by colonization of endophytic fungus Hendersonia toruloidea. Frontiers in Plant Science, 8. pp. 1-8. ISSN 1664-462X


Thiamine, or vitamin B1 plays an indispensable role as a cofactor in crucial metabolic reactions including glycolysis, pentose phosphate pathway and the tricarboxylic acid cycle in all living organisms. Thiamine has been shown to play a role in plant adaptation toward biotic and abiotic stresses. The modulation of thiamine biosynthetic genes in oil palm seedlings was evaluated in response to root colonization by endophytic Hendersonia toruloidea. Seven-month-old oil palm seedlings were inoculated with H. toruloidea and microscopic analyses were performed to visualize the localization of endophytic H. toruloidea in oil palm roots. Transmission electron microscopy confirmed that H. toruloidea colonized cortical cells. The expression of thiamine biosynthetic genes and accumulation of total thiamine in oil palm seedlings were also evaluated. Quantitative real-time PCR was performed to measure transcript abundances of four key thiamine biosynthesis genes (THI4, THIC, TH1, and TPK) on days 1, 7, 15, and 30 in response to H. toruloidea colonization. The results showed an increase of up to 12-fold in the expression of all gene transcripts on day 1 post-inoculation. On days 7, 15, and 30 post-inoculation, the relative expression levels of these genes were shown to be downregulated. Thiamine accumulation was observed on day 7 post-colonization and subsequently decreased until day 30. This work provides the first evidence for the enhancement of thiamine biosynthesis by endophytic colonization in oil palm seedlings.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.3389/fpls.2017.01799
Publisher: Frontiers Media
Keywords: Oil palm; Endophytic fungi; Thiamine biosynthesis; Gene expression; Endophytic colonization
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 11 Jan 2019 03:41
Last Modified: 11 Jan 2019 03:41
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3389/fpls.2017.01799
URI: http://psasir.upm.edu.my/id/eprint/61731
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