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Determination of Poly-β-Hydroxyalkanoate in peat


Dowrick, David J. and Md Yusoff, Fatimah and Gough, Rachel and Freeman, Christopher (2017) Determination of Poly-β-Hydroxyalkanoate in peat. Communications in Soil Science and Plant Analysis, 48 (5). 576 - 580. ISSN 0010-3624; ESSN: 1532-2416


Poly-β-hydroxyalkanoate (PHA) is a prokaryotic energy reserve material that has been used as an indicator of environmental stress in aquatic bacteria. The following technique has been used to quantify PHA in peatland microorganisms. Peat samples were dried, digested in sulphuric acid to convert PHA into crotonic acid, and the resulting acid determined using organic acid high performance liquid chromatography (HPLC) and ultraviolet (UV) detection at 214 nm. This technique is suggested to have potential value as an indicator of environmental stress on peatland microorganisms, such as that caused by summer drought, or changes in soil nutrient availability, which are predicted consequences of climatic change.

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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.1080/00103624.2017.1282506
Publisher: Taylor & Francis
Keywords: High performance liquid chromatography; Microbes; Peatland; Poly-β-hydroxyalkanoate
Depositing User: Nurul Ainie Mokhtar
Date Deposited: 23 May 2018 00:36
Last Modified: 23 May 2018 00:36
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/00103624.2017.1282506
URI: http://psasir.upm.edu.my/id/eprint/61429
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