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Cloning, production and characterization of a glycoside hydrolase family 7 enzyme from the gut microbiota of the termite Coptotermes curvignathus


Citation

Woon, James Sy-Keen and King, Patricia Jie Hung and Mackeen, Mukram Mohamed and Mahadi, Nor Muhammad and Wan Seman, Wan Mohd Khairulikhsan and Broughton, William J. and Abdul Murad, Abdul Munir and Abu Bakar, Farah Diba (2017) Cloning, production and characterization of a glycoside hydrolase family 7 enzyme from the gut microbiota of the termite Coptotermes curvignathus. Molecular Biotechnology, 59 (7). 271 - 283. ISSN 1073-6085; ESSN: 1559-0305

Abstract

Coptotermes curvignathus is a termite that, owing to its ability to digest living trees, serves as a gold mine for robust industrial enzymes. This unique characteristic reflects the presence of very efficient hydrolytic enzyme systems including cellulases. Transcriptomic analyses of the gut of C. curvignathus revealed that carbohydrate-active enzymes (CAZy) were encoded by 3254 transcripts and that included 69 transcripts encoding glycoside hydrolase family 7 (GHF7) enzymes. Since GHF7 enzymes are useful to the biomass conversion industry, a gene encoding for a GHF7 enzyme (Gh1254) was synthesized, sub-cloned and expressed in the methylotrophic yeast Pichia pastoris. Expressed GH1254 had an apparent molecular mass of 42 kDa, but purification was hampered by its low expression levels in shaken flasks. To obtain more of the enzyme, GH1254 was produced in a bioreactor that resulted in a fourfold increase in crude enzyme levels. The purified enzyme was active towards soluble synthetic substrates such as 4-methylumbelliferyl-β-d-cellobioside, 4-nitrophenyl-β-d-cellobioside and 4-nitrophenyl-β-d-lactoside but was non-hydrolytic towards Avicel or carboxymethyl cellulose. GH1254 catalyzed optimally at 35 °C and maintained 70% of its activity at 25 °C. This enzyme is thus potentially useful in food industries employing low-temperature conditions.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Agriculture and Food Sciences
DOI Number: https://doi.org/10.1007/s12033-017-0015-x
Publisher: Humana Press
Keywords: Avicel; Biomass; Bioreactor; Cellulase; Saccharification; Symbiont
Depositing User: Nida Hidayati Ghazali
Date Deposited: 14 Sep 2018 03:27
Last Modified: 14 Sep 2018 03:27
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s12033-017-0015-x
URI: http://psasir.upm.edu.my/id/eprint/61129
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