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Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex


Citation

Ashwaq, Al-Abboodi Shakir and Abdullah, Rasedee @ Mat and Abdul, Ahmad Bustamam and Yap, Taufiq Yun Hin and Al-Qubaisi, Mothanna Sadiq and Eid, Eltayeb E. M. (2017) Characterization, drug release profile and cytotoxicity of dentatin-hydroxypropyl-β-cyclodextrin complex. Journal of Inclusion Phenomena and Macrocyclic Chemistry, 87. 167 - 178. ISSN 1388-3127

Abstract / Synopsis

This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into Hydroxypropyl-β-Cyclodextrin (HPβCD) cavity. To confirm that inclusion be succeeded, the produced complex were installed onto different machines. The latter includes: Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and field emission-scanning electron microscopy (FE-SEM). The hydrodynamic diameter and zeta potential of DEN-HPβCD complex were 2.025 ± 0.39 nm and −33.6 mV, respectively. Ultra-violet spectroscopy was employed to further confirmation of complexation process as well as to determine drug release profile. The result showed an initial burst release (19.9% within first two minutes) and then a continuous release for an extended period of 41 h (100%). The solubility of DEN was enhanced by >300 fold following complexation when a compared to DEN alone. Moreover, MTT finding showed that this complexation did not reduce cytotoxicity of DEN after applying on prostate cancer (LNCaP), human adenocarcinoma breast cancer (MDA-MB-231) and human gastric adenocarcinoma cell line (HDT). However, further investigations are required to validate efficacy of our produced inclusion using molecular analysis and in vivo studies.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Faculty of Science
Faculty of Veterinary Medicine
Institute of Bioscience
DOI Number: https://doi.org/10.1007/s10847-016-0688-y
Publisher: Springer Netherlands
Keywords: Dentatin; Hydroxypropyl-β-cyclodextrin; Physical properties; Solubility; Drug release
Depositing User: Nida Hidayati Ghazali
Date Deposited: 20 Sep 2018 12:04
Last Modified: 20 Sep 2018 12:04
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1007/s10847-016-0688-y
URI: http://psasir.upm.edu.my/id/eprint/61100
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