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Changes in the expressions of thiamine biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaies guineensis) as response to salinity stress


Citation

Abdul Rahman, Nur Syuhadah and Ghazali, Nur Sakinah and Balia Yusof, Zetty Norhana (2017) Changes in the expressions of thiamine biosynthesis genes (THIC and THI1/THI4) in oil palm (Elaies guineensis) as response to salinity stress. Research on Crops, 18 (4). 634 - 641. ISSN 0972-3226; ESSN: 2348-7542

Abstract / Synopsis

Thiamine or also known as vitamin B 1 , it is the first water soluble B-complex vitamin to be identified which plays an important role as both cofactor and non-cofactor. Its active form, thiamine pyrophosphate (TPP) is important as a cofactor for various types of enzymes involved in central metabolism. Besides that, thiamine has been shown to have a role in plant protection against stress. In this study, we investigated the expressions of the first two enzymes involved in thiamine biosynthesis pathway, THIC and THI1/THI4 when subjected to salinity stress induced by sodium chloride (NaCl) at various concentrations, namely, 0, 50, 150 and 200 mM. RT-PCR was conducted to amplify both gene transcripts and the intensities of the bands were analyzed by using the ImageJ software. Overall, there was an increase in expression for both THIC and THI1/THI4 gene transcripts upon stress application. For THIC, the highest level of expression was observed on day 7 for 50 mM NaCl treated oil palm seedling with an increase of up to 331%, while for THI1/THI4, the highest level of expression was 723% on day 3 when 200 mM NaCl was applied to the oil palm seedlings. These results obtained are in agreement with suggestions that thiamine plays an additional role in plant protection against stress as it may lead to an overexpression of thiamine in general.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.5958/2348-7542.2017.00106.1
Publisher: Gaurav Society of Agricultural Research Information Centre
Keywords: Abiotic stress; Gene expression; Oil palm; Salinity stress; Thiamine; Vitamin B1
Depositing User: Nida Hidayati Ghazali
Date Deposited: 31 Oct 2018 16:24
Last Modified: 31 Oct 2018 16:24
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.5958/2348-7542.2017.00106.1
URI: http://psasir.upm.edu.my/id/eprint/61077
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