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Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model


Citation

Lee, Yu Zhao and Yap, Hui Min and Shaari, Khozirah and Tham, Chau Ling and Sulaiman, Mohd Roslan and Israf Ali, Daud Ahmad (2017) Blockade of eosinophil-induced bronchial epithelial-mesenchymal transition with a geranyl acetophenone in a coculture model. Frontiers in Pharmacology, 8. pp. 1-13. ISSN ESSN: 1663-9812

Abstract

Epithelial-mesenchymal transition (EMT) is currently recognized as the main cellular event that contributes to airway remodeling. Eosinophils can induce EMT in airway epithelial cells via increased transforming growth factor (TGF)-β production. We assessed the effect of synthetic 2,4,6-trihydroxy-3-geranyl acetophenone (tHGA) upon eosinophil-induced EMT in a cellular model. The human eosinophil cell line EoL-1 was used to induce EMT in BEAS-2B human bronchial epithelial cells. The induction of EMT was dose-dependently suppressed following tHGA treatment in which the epithelial morphology and E-cadherin expression were not altered. Protein and mRNA expression of vimentin, collagen I and fibronectin in eosinophil-induced epithelial cells were also significantly suppressed by tHGA treatment. Following pathway analysis, we showed that tHGA suppressed eosinophil-induced activator protein-1-mediated TGF-β production by targeting c-Jun N-terminal kinase and phosphoinositide 3-kinase signaling pathways. These findings corroborated previous findings on the ability of tHGA to inhibit experimental murine airway remodeling.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
Institute of Bioscience
DOI Number: https://doi.org/10.3389/fphar.2017.00837
Publisher: Frontiers Research Foundation
Keywords: Geranyl acetophenone; Epithelial-mesenchymal transition; Transforming growth factor beta; Airwayremodeling; Asthma
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 30 Apr 2019 03:45
Last Modified: 30 Apr 2019 03:45
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3389/fphar.2017.00837
URI: http://psasir.upm.edu.my/id/eprint/61018
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