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Biosynthesis of ZnO nanoparticles by a new yeast strain Pichia kudriavzevii and evaluation of their antimicrobial and antioxidant activities


Citation

Amin, Boroumand Moghaddam and Moniri, Mona and Azizi, Susan and Abdul Rahim, Raha and Ariff, Arbakariya and Saad, Wan Zuhainis and Namvar, Farideh and Navaderi, Mohammad and Mohamad, Rosfarizan (2017) Biosynthesis of ZnO nanoparticles by a new yeast strain Pichia kudriavzevii and evaluation of their antimicrobial and antioxidant activities. Molecules, 22 (6). pp. 1-18. ISSN 1420-3049

Abstract / Synopsis

The potential ability of a new yeast strain, Pichia kudriavzevii, in the synthesis of zinc oxide nanoparticles (ZnO-NPs) through a green method was explored in this study. The effect of reaction time (12, 24 and 36 h) on the structure of the resulting ZnO nanoparticles was investigated. From the XRD and TEM results, the ZnO-NPs with a hexagonal wurtzite structure and a particle crystal size of ~10-61 nm was formed at different reaction times. Combing XRD, TEM, and PL results, it was revealed that the sample prepared at intermediate duration (24 h) has the most favorable nanosized structure with the lowest defect concentration. The biomedical properties of ZnO-NPs as free radical scavenging activity, cytotoxicity and antibacterial agents were characterized. Biosynthesized ZnO-NPs showed strong DPPH free radical scavenging and a dose dependent toxicity with non-toxic effects on Vero cells for concentrations below 190 µg/mL. Desirable bactericidal activity was shown by the ZnO-NPs on Gram-positive bacteria (Bacillus subtilis, Staphylococcus epidermidis and Staphylococcus aurous) and Gram-negative bacteria (Escherichia coli and Serratia marcescens). A maximum inhibition zone of ~19 mm was observed for Staphylococcus epidermidis at a concentration of 100 µg/mL for sample prepared at 24 h. The results from this study reveal that ZnO-NPs possesses potential for many medical and industrial applications.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Tropical Forestry and Forest Products
DOI Number: https://doi.org/10.3390/molecules22060872.
Publisher: MDPI
Keywords: Pichia kudriavzevii; Antibacterial activity; Antioxidant; Green synthesis; Zinc oxide nanoparticles
Depositing User: Nida Hidayati Ghazali
Date Deposited: 20 Mar 2019 14:14
Last Modified: 20 Mar 2019 14:14
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/molecules22060872.
URI: http://psasir.upm.edu.my/id/eprint/61010
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