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Biosensor based on tyrosinase immobilized on graphene decorated gold nanoparticle/chitosan for phenolic detection in aqueous


Citation

Mohamed Fartas, Fuzi and Abdullah, Jaafar and Yusof, Nor Azah and Sulaiman, Yusran and Saiman, Mohd Izham (2017) Biosensor based on tyrosinase immobilized on graphene decorated gold nanoparticle/chitosan for phenolic detection in aqueous. Sensors, 17 (5). pp. 1-14. ISSN 1424-8220

Abstract

In this research work, electrochemical biosensor was fabricated based on immobilization of tyrosinase onto graphene-decorated gold nanoparticle/chitosan (Gr-Au-Chit/Tyr) nanocomposite-modified screen-printed carbon electrode (SPCE) for the detection of phenolic compounds. The nanocomposite film was constructed via solution casting method. The electrocatalytic activity of the proposed biosensor for phenol detection was studied using differential pulse voltammetry (DPV) and cyclic voltammetry (CV). Experimental parameters such as pH buffer, enzyme concentration, ratio of Gr-Au-Chit, accumulation time and potential were optimized. The biosensor shows linearity towards phenol in the concentration range from 0.05 to 15 μM with sensitivity of 0.624 μA/μM and the limit of detection (LOD) of 0.016 μM (S/N = 3). The proposed sensor also depicts good reproducibility, selectivity and stability for at least one month. The biosensor was compared with high-performance liquid chromatography (HPLC) method for the detection of phenol spiked in real water samples and the result is in good agreement and comparable.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Institute of Advanced Technology
DOI Number: https://doi.org/10.3390/s17051132
Publisher: Multidisciplinary Digital Publishing Institute
Keywords: Tyrosinase; Graphene-gold nanocomposite; Electrochemical biosensor; Phenoliccompounds
Depositing User: Nida Hidayati Ghazali
Date Deposited: 30 Apr 2019 03:20
Last Modified: 30 Apr 2019 03:20
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3390/s17051132
URI: http://psasir.upm.edu.my/id/eprint/61007
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