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A transmission electron microscopy study of anticoagulant-induced platelet vesiculation


Almhanawai, Bahaa Hadi Jaber and Khalid, Bahariah and Tengku Ibrahim, Tengku Azmi and Mohd Tohit, Eusni Rahayu (2017) A transmission electron microscopy study of anticoagulant-induced platelet vesiculation. Porto Biomedical Journal, 2 (1). 23 - 27. ISSN 2444-8664


Platelet microparticles (PMPs) are small membrane fragments released from activated platelets in response to various stimuli. PMPs serve as biomarkers for several diseases and conditions and are useful tools for prognostic, diagnostic, and therapeutic purposes. The objective of our study was to compare the direct effects of ethylenediaminetetraacetic acid (EDTA) and sodium citrate anticoagulants on platelet structure and PMP vesiculation using transmission electron microscopy to visualize the morphologic changes in platelets. Micrographs revealed that platelets in the EDTA-anticoagulated tube manifested with significant morphologic changes and induced PMP vesiculation. On the other hand, the sodium citrate-anticoagulated tube showed a normal platelet structure and minor modifications in some cases, with poor indication of PMP vesiculation. In conclusion, EDTA induced platelet activation and PMP vesiculation and represents a major source of artifacts during the pre-analysis steps of PMP vesiculation.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1016/j.pbj.2016.11.002
Publisher: PBJ-Associaco Porto Biomedical
Keywords: EDTA; Platelet microparticles; Pre-analytical artifacts; Sodium citrate; Transmission electron microscopy
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 29 Apr 2019 01:17
Last Modified: 29 Apr 2019 01:17
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.pbj.2016.11.002
URI: http://psasir.upm.edu.my/id/eprint/60752
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