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A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus


Citation

Nordin, Noordiana and Yusof, Nor Azah and Abdullah, Jaafar and Radu, Son and Hushiarian, Roozbeh (2017) A simple, portable, electrochemical biosensor to screen shellfish for vibrio parahaemolyticus. AMB Express, 7 (41). pp. 1-9. ISSN 2191-0855

Abstract

An earlier electrochemical mechanism of DNA detection was adapted and specified for the detection of Vibrio parahaemolyticus in real samples. The reader, based on a screen printed carbon electrode, was modified with polylactide-stabilized gold nanoparticles and methylene blue was employed as the redox indicator. Detection was assessed using a microprocessor to measure current response under controlled potential. The fabricated sensor was able to specifically distinguish complementary, non-complementary and mismatched oligonucleotides. DNA was measured in the range of 2.0 × 10−8–2.0 × 10−13 M with a detection limit of 2.16 pM. The relative standard deviation for 6 replications of differential pulse voltammetry (DPV) measurement of 0.2 µM complementary DNA was 4.33%. Additionally, cross-reactivity studies against various other food-borne pathogens showed a reliably sensitive detection of the target pathogen. Successful identification of Vibrio parahaemolyticus (spiked and unspiked) in fresh cockles, combined with its simplicity and portability demonstrate the potential of the device as a practical screening tool.


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Additional Metadata

Item Type: Article
Divisions: Institute of Advanced Technology
DOI Number: https://doi.org/10.1186/s13568-017-0339-8
Publisher: Springer Open
Keywords: Vibrio parahaemolyticus; Food-borne pathogens; Electrochemical DNA sensor; Shellfish; Portable biosensor
Depositing User: Nida Hidayati Ghazali
Date Deposited: 28 Mar 2019 04:49
Last Modified: 28 Mar 2019 04:49
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/s13568-017-0339-8
URI: http://psasir.upm.edu.my/id/eprint/60735
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