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A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery


Citation

Biabanikhankahdani, Roya and Bayat, Saadi and Ho, Kok Lian and Mohammed Alitheen, Noorjahan Banu and Tan, Wen Siang (2017) A simple add-and display method for immobilisation of cancer drug on his-tagged virus-like nanoparticles for controlled drug delivery. Scientific Reports, 7. pp. 1-12. ISSN 2045-2322

Abstract

pH-responsive virus-like nanoparticles (VLNPs) hold promising potential as drug delivery systems for cancer therapy. In the present study, hepatitis B virus (HBV) VLNPs harbouring His-tags were used to display doxorubicin (DOX) via nitrilotriacetic acid (NTA) conjugation. The His-tags served as pH-responsive nanojoints which released DOX from VLNPs in a controlled manner. The His-tagged VLNPs conjugated non-covalently with NTA-DOX, and cross-linked with folic acid (FA) were able to specifically target and deliver the DOX into ovarian cancer cells via folate receptor (FR)-mediated endocytosis. The cytotoxicity and cellular uptake results revealed that the His-tagged VLNPs significantly increased the accumulation of DOX in the ovarian cancer cells and enhanced the uptake of DOX, which improved anti-tumour effects. This study demonstrated that NTA-DOX can be easily displayed on His-tagged VLNPs by a simple Add-and-Display step with high coupling efficiency and the drug was only released at low pH in a controlled manner. This approach facilitates specific attachment of any drug molecule on His-tagged VLNPs at the very mild conditions without changing the biological structure and native conformation of the VLNPs.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Medicine and Health Science
Faculty of Science
DOI Number: https://doi.org/10.1038/s41598-017-05525-4
Publisher: Nature Publishing Group
Keywords: Virus-like nanoparticles (VLNPs); Cancer drug; Drug therapy
Depositing User: Ms. Nida Hidayati Ghazali
Date Deposited: 28 Mar 2019 04:22
Last Modified: 28 Mar 2019 04:22
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1038/s41598-017-05525-4
URI: http://psasir.upm.edu.my/id/eprint/60732
Statistic Details: View Download Statistic

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