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SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest


Tukiran, Nur Azira and Mohamed Roslan, Nur Illiyin and Ismail, Amin (2014) SDS-PAGE and enzyme immunoassay methods for detection of porcine gelatin in edible bird's nest. In: Malaysia International Halal Research & Education Conference 2014 (MIHREC 2014), 2-4 Dec. 2014, Marriott Putrajaya Hotel, Malaysia. (pp. 34-39).


Porcine gelatin is one of the common adulterant found in processed edible bird’s nest (EBN); as to increase its net weight prior to sale. This study aimed to develop a sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and an enzyme immunoassay method to detect the presence of porcine gelatin in processed EBN. SDS-PAGE method was developed by comparing the polypeptide patterns of porcine gelatin with the EBN. In the evaluation of the spiked samples of 2.5% to 50% (v/v), SDS-PAGE was able to detect 20% of porcine gelatin in EBN. An indirect enzyme linked immunosorbent assay (ELISA) was then developed for detection of lower amounts of porcine gelatin. A polyclonal rabbit antibodies was raised against the species-specific sequence of porcine collagen alpha 2 (I) chain. The detection limit for porcine gelatin was 0.05% in EBN. This ELISA is suitable for monitoring of EBN’s quality and purity in the supply chain.

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Additional Metadata

Item Type: Conference or Workshop Item (Other)
Divisions: Faculty of Medicine and Health Science
Halal Products Research Institute
Publisher: Halal Products Research Institute, Universiti Putra Malaysia
Keywords: Edible bird's nest; Porcine gelatin; Adulteration; ELISA; SDS-PAGE
Depositing User: Nabilah Mustapa
Date Deposited: 07 Jun 2018 06:24
Last Modified: 07 Jun 2018 06:24
URI: http://psasir.upm.edu.my/id/eprint/60502
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