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Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality


Citation

Abu, Nadiah and Yeap, Swee Keong and Nordin, Noraini and Tan, Sheau Wei and Ho, Wan Yong and Mohammed Alitheen, Noorjahan Banu (2018) Optimization of illumina TruSeq Targeted RNA expression (TREx) library quality. Sains Malaysiana, 47 (2). pp. 303-308. ISSN 0126-6039

Abstract

RNA-seq has become an essential tool in molecular research. Nevertheless, application of RNA-seq was limited by cost and technical difficulties. Illumina has introduced the cost effective and ease to handle Truseq Targeted RNA Sequencing. In this study, we present the requirements and the optimization procedure for this Truseq Targeted RNA sequencing on cell line. Total RNA was recommended as starting materials but it required optimization including additional purification step and adjusting the AMPure beads ratio to eliminate unwanted contaminants. This can be resolved by using PolyA-enriched mRNA as starting material. TREx is a useful assay to evaluate gene expression. Quality library of TREx can be prepared by adding multiple washing steps or changing input sample to mRNA.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Institute of Bioscience
DOI Number: https://doi.org/10.17576/jsm-2018-4702-12
Publisher: Penerbit Universiti Kebangsaan Malaysia
Keywords: Illumina; Poly(A) mRNA; Targeted expression; TREx
Depositing User: Nabilah Mustapa
Date Deposited: 21 May 2018 03:51
Last Modified: 21 May 2018 03:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.17576/jsm-2018-4702-12
URI: http://psasir.upm.edu.my/id/eprint/60474
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