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Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products


Citation

Tang, Hooi Chia and Sieo, Chin Chin and Abdullah, Norhani and Mohamad, Rosfarizan and Omar, Abdul Rahman and Chong, Chun Wie and Fitrianto, Anwar and Ho, Yin Wan (2018) Production of phytase by Mitsuokella jalaludinii in semi-solid state fermentation of agricultural by-products. Sains Malaysiana, 47 (2). pp. 277-286. ISSN 0126-6039

Abstract

Phytase activity and growth of anaerobic rumen bacterium, Mitsuokella jalaludinii were investigated by semi-solid state fermentation. Carbon source (rice bran, yam and cassava), nitrogen sources (soya bean, offal meal, fish meal and feather meal) and growth factors (hemin, L-cysteine hydrochloride and minerals) were evaluated in a one-factor-at-a-time approach. Rice bran and fish meal produced better growth and phytase enzyme activity. The removal of L-cysteine hydrochloride and minerals significantly decreased (p<0.05) phytase activity from 1178.72 U to 446.99 U and 902.54 U, respectively. The response surface methods (RSM) was conducted to optimize the phytase production and the results showed the combination of 7.7% of rice bran and 3.7% of fish meal in semi-solid state fermentation gave the highest phytase activity. Maximum phytase production and optimum growth of bacteria were detected at 12 h incubation in both MF medium (control) and agro-medium. In this agro-medium, M. jalaludinii produced 2.5 fold higher phytase activity compared to MF medium.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Science
Institute of Bioscience
Institute of Tropical Agriculture and Food Security
DOI Number: https://doi.org/10.17576/jsm-2018-4702-09
Publisher: Penerbit Universiti Kebangsaan Malaysia
Keywords: Agro-medium; Mitsuokella jalaludinii; Phytase activity; Response surface methods; Semi-solid state fermentation
Depositing User: Nabilah Mustapa
Date Deposited: 21 May 2018 03:51
Last Modified: 21 May 2018 03:51
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.17576/jsm-2018-4702-09
URI: http://psasir.upm.edu.my/id/eprint/60473
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