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Sperm DNA impairment in the bull: causes, influences on reproduction and evaluations


Citation

Baiee, Falah Hasan Ali and Haron, Abd Wahid and Yusoff, Rosnina and Omar, Mohamed Ariff and Degu, Nurhusien Yimer (2018) Sperm DNA impairment in the bull: causes, influences on reproduction and evaluations. Pertanika Journal of Tropical Agricultural Science, 41 (1). pp. 63-80. ISSN 1511-3701; ESSN: 2231-8542

Abstract

Conventional semen examination involving sperm motility, viability and morphology remains the backbone of assessing the fertility status of a sire. However, there remains instances where these semen parameters appear normal but cases of low conception rates or failure of pregnancy occur. This review highlights the causes of sperm DNA damage and the effectiveness of techniques designed to evaluate the contribution of sperm DNA damage to lowered fertility in bulls. Among the many causes of sperm DNA impairment are imperfect spermatogenesis, faulty apoptosis, reactive oxygen species, in-vitro handling, impact of environment, radiography and the stress of cryopreservation processes. Furthermore, DNA impairment impairs fertilisation, interferes with embryonic development and implantation and blocks blastocyst formation. The most frequently used tests to determine DNA damage are the acridine orange test (AOT) using acridine orange stain with examination under a fluorescence microscope and the sperm chromatin structure assay (SCSA) using the same stain but examined with flow cytometry.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
Publisher: Universiti Putra Malaysia Press
Keywords: Sperm; DNA impairment; Sperm DNA evaluation
Depositing User: Nabilah Mustapa
Date Deposited: 12 Mar 2018 07:48
Last Modified: 12 Mar 2018 07:48
URI: http://psasir.upm.edu.my/id/eprint/59604
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