UPM Institutional Repository

Comparative ultrastructural hepatic alterations induced by free and liposome-encapsulated mefenamic acid


Citation

Jarrar, Qais Bashir Mahmoud and Abdullah, Muhammad Nazrul Hakim and Cheema, Manraj Singh and Zakaria, Zainul Amiruddin (2017) Comparative ultrastructural hepatic alterations induced by free and liposome-encapsulated mefenamic acid. Ultrastructural Pathology, 41 (5). pp. 335-345. ISSN 0191-3123; ESSN: 1521-0758

Abstract

Mefenamic acid (MFA) is used as an anti-inflammatory, antinociceptive, and antipyretic agent for treatment of a wide range of pathological disorders. While the uncertainty of its safety and the poor oral bioavailability constitute the major limiting factors of its medical use, considerable efforts including liposomal encapsulation are needed to achieve maximum therapeutic advantages. The current work was conducted to investigate the ultrastructural alterations in the liver induced by free MFA and its liposomal preparation. Female Sprague-Dawley rats were treated with daily oral doses of either free MFA or MFA entrapped in Tween 80 inoculated liposomes at the concentration of 80 mg/kg for 28 days. Ultrathin sections were prepared from biopsies taken from the liver of each member of all animals under study and subjected to examination by transmission electron microscopy. The liver of rats that were exposed to liposomal MFA showed more ultrastructural alterations than the rats treated with the free drug. While both groups of rats demonstrated sinusoidal dilatation, Kupffer cell hyperplasia, mitochondrial damage, and nuclear alterations, rats treated with liposome-encapsulated MFA induced an increase in the multiple lysosomes formation, hepatocytic steatosis, and apoptotic activity than free MFA-treated rats. The ultrastructural findings of the present study indicate that the use of liposomal MFA induces more hepatic damage than the use of free MFA.


Download File

[img]
Preview
Text (Abstract)
Comparative ultrastructural hepatic alterations induced by free and liposome-encapsulated mefenamic acid.pdf

Download (35kB) | Preview

Additional Metadata

Item Type: Article
Divisions: Faculty of Medicine and Health Science
DOI Number: https://doi.org/10.1080/01913123.2017.1349850
Publisher: Taylor & Francis
Keywords: Drug delivery; Hepatocytes; Liposome; Mefenamic acid; Toxicity; Ultrastructural alterations
Depositing User: Nabilah Mustapa
Date Deposited: 01 Mar 2018 03:21
Last Modified: 01 Mar 2018 03:21
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1080/01913123.2017.1349850
URI: http://psasir.upm.edu.my/id/eprint/59393
Statistic Details: View Download Statistic

Actions (login required)

View Item View Item