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Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension


Citation

Karim, Mohd Shahrul Nizwanshah and Abdullah, Siti Nor Akmar (2017) Mutagenesis of the promoter of the oil palm homogentisate geranylgeranyl transferase gene (HGGT) by PCR-driven overlap extension. In: International Conference on Big Data Applications in Agriculture (ICBAA2017), 5-6 Dec. 2017, Auditorium Putra, TNCPI Building, Universiti Putra Malaysia. (pp. 191-195).

Abstract

PCR-driven overlap extension is a simple and versatile tool in performing site-directed mutagenesis. This technique contains two stages of PCR. The first stage is the initial PCR to generate mutated gene segments that overlapped, which are then used as a DNA template in the second stage PCR to produce full length product with the site-directed mutation. The internal primer serves to introduce nucleotide substitution for site-directed mutagenesis by complementary 3’ ends on the intermediate segments. The full-length product amplied by the flanking primers in the second stage PCR includes the restriction enzyme sites for inserting the product into an expression vector for cloning purposes.


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Additional Metadata

Item Type: Conference or Workshop Item (Poster)
Divisions: Institute of Plantation Studies
Publisher: Institute of Plantation Studies, Universiti Putra Malaysia
Keywords: Mutagenesis; Primer; PCR; Oil palm
Depositing User: Nabilah Mustapa
Date Deposited: 07 Feb 2018 03:40
Last Modified: 07 Feb 2018 03:40
URI: http://psasir.upm.edu.my/id/eprint/58843
Statistic Details: View Download Statistic

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