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The operation performance of an expanded bed contactor characterised by mechanical stirring


Citation

Ling, Tau Chuan and Lan, John Chi Wei and Lyddiatt, Andrew (2006) The operation performance of an expanded bed contactor characterised by mechanical stirring. Asia Pacific Journal of Molecular Biology and Biotechnology, 14 (1). pp. 27-31. ISSN 0128-7451

Abstract

The performance of an expanded bed contactor (UpFront i.d. 20 mm) characterised by mechanical stirring flow distribution in the adsorption of intracellular proteins from concentrated unclarified yeast extract was investigated. High density pellicular adsorbent (UpFront steel-agarose; ρ = 2.65 g ml-1) derivatized with selective ligand chemistries (Cibacron Blue 3GA) was adopted in this study. The adsorption of glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from bakers’ yeast was chosen as a demonstration of this approach. It was demonstrated that a high biomass throughput adsorption operation (25 % ww/v of yeast extract) was achieved in this contactor design.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Publisher: Malaysian Society for Molecular Biology and Biotechnology
Keywords: Expanded bed contactor; Mechanical stirring; Flow distribution; Yeast extract; G3PDH
Depositing User: Nabilah Mustapa
Date Deposited: 18 Jan 2018 10:15
Last Modified: 18 Jan 2018 10:15
URI: http://psasir.upm.edu.my/id/eprint/58496
Statistic Details: View Download Statistic

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