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The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract


Citation

Ling, Tau Chuan and Lyddiatt, Andrew (2005) The comparative recovery performance of anion exchange and dye-ligand fluidised bed adsorption of G3PDH from unclarified yeast extract. Asia Pacific Journal of Molecular Biology and Biotechnology, 13 (1). pp. 59-62. ISSN 0128-7451

Abstract

The comparative recovery performance of anion exchange and dye ligand fluidised bed adsorption of intracellular enzyme, glyceraldehydes 3-phosphate dehydrogenase (G3PDH) from unclarified disrupted yeast has been undertaken. The commercially available anion exchanger, Streamline QXL and the kiesleguhr-agarose composite adsorbent, Microsorb K6AX derived with dye-ligand (Cibaron Blue 3GA) were employed in fluidized bed experiments. The adsorbents were evaluated in respect of recovery performance in terms of yield, purity and enzyme specific activity.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Publisher: Malaysian Society for Molecular Biology and Biotechnology
Keywords: Anion exchange; Dye-ligand; Cibacron Blue 3GA; Fluidised bed adsorption; G3PDH
Depositing User: Nabilah Mustapa
Date Deposited: 18 Jan 2018 09:54
Last Modified: 18 Jan 2018 09:54
URI: http://psasir.upm.edu.my/id/eprint/58492
Statistic Details: View Download Statistic

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