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Evaluation of in vitro wound healing efficacy of breadfruit derived starch hydrolysate


Mohamed Amin, Zahiah and Koh, Soo Peng and Tan, Chin Ping and Yeap, Swee Keong and Abdul Hamid, Nur Syazwani and Long, Kamariah (2017) Evaluation of in vitro wound healing efficacy of breadfruit derived starch hydrolysate. International Food Research Journal, 24 (4). pp. 1771-1781. ISSN 1985-4668; ESSN: 2231-7546


To study the wound healing efficacy of breadfruit starch hydrolysate, an in vitro wound scratch assay was conducted, in which the migration rate of wounded NIH 3T3 fibroblasts was determined. Wounds treated with lower dextrose equivalent (DE), (DE 10-14) starch hydrolysate were found capable to improve the wound healing of NIH 3T3 fibroblast cell with the percentage of wound closure improvement of 77%, respectively when compared with higher DE range (DE 15-19 and DE 20-24). The findings obtained in the BrdU uptake and MTT viability assays confirmed the wound healing properties of breadfruit starch hydrolysate as the starch hydrolysate-treated wounded NIH 3T3 fibroblasts were able to proliferate well and no cytotoxicity was observed. Together, these findings indicated that the newly developed breadfruit starch hydrolysate performed better than commercial (COM) starch hydrolysate of the same DE ranges. In conclusion, breadfruit starch hydrolysate had better functional properties than did starch hydrolysates derived from other sources and that they could play a beneficial role in wound healing applications.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Food Science and Technology
Institute of Bioscience
Publisher: Faculty of Food Science and Technology, Universiti Putra Malaysia
Keywords: Breadfruit; Starch hydrolysate; Wound closure; Enzymatic processing; NIH 3T3 cell line
Depositing User: Nabilah Mustapa
Date Deposited: 12 Jan 2018 08:17
Last Modified: 12 Jan 2018 08:17
URI: http://psasir.upm.edu.my/id/eprint/58367
Statistic Details: View Download Statistic

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