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Scale-up synthesis of lipase-catalyzed palm esters in stirred-tank reactor


Keng, Pei Sin and Basri, Mahiran and Ariff, Arbakariya and Abdul Rahman, Mohd Basyaruddin and Raja Abdul Rahman, Raja Noor Zaliha and Salleh, Abu Bakar (2008) Scale-up synthesis of lipase-catalyzed palm esters in stirred-tank reactor. Bioresource Technology, 99 (14). pp. 6097-6104. ISSN 0960-8524; ESSN: 1873-2976


Lipase-catalyzed production of palm esters by alcoholysis of palm oil with oleyl alcohol in n-hexane was performed in 2 L stirred-tank reactor (STR). Investigation on the performance of reactor operation was carried out in batch mode STR with single impeller mounted on the centrally located shaft. Rushton turbine (RT) impellers provide the highest reaction yield (95.8%) at lower agitation speed as compared to AL-hydrofoil (AL-H) and 2-bladed elephant ear (EE) impellers. Homogenous enzyme particles suspension was obtained at 250 rpm by using RT impeller. At higher impeller speed, the shear effect on the enzyme particles caused by agitation has decreased the reaction performance. Palm esters reaction mixture in STR follows Newtons’ law due to the linear relation between the shear stress (s) and shear rate (dt/dy). High stability of Lipozyme RM IM was observed as shown by its ability to be repeatedly used to give high percentage yield (79%) of palm esters even after 15 cycles of reaction. The process was successfully scale-up to 75 L STR (50 L working volume) based on a constant impeller tip speed approach, which gave the yield of 97.2% after 5 h reaction time.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Science
Faculty of Biotechnology and Biomolecular Sciences
DOI Number: https://doi.org/10.1016/j.biortech.2007.12.049
Publisher: Elsevier
Keywords: Palm esters; Stirred-tank reactor; Single impeller; Enzyme suspension; Operational stability
Depositing User: Najwani Amir Sariffudin
Date Deposited: 29 Apr 2010 10:23
Last Modified: 28 Sep 2016 02:15
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.biortech.2007.12.049
URI: http://psasir.upm.edu.my/id/eprint/5805
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