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Biophysical properties of DNA in hydrated ionic liquids


Jumbri, Khairulazhar and Ahmad, Haslina and Abd. Malek, Emilia and Abdul Rahman, Mohd Basyaruddin (2016) Biophysical properties of DNA in hydrated ionic liquids. In: 4th International Conference of Fundamental and Applied Sciences (ICFAS 2016), 15-17 Aug. 2016, Kuala Lumpur Convention Centre, Kuala Lumpur. (pp. 1-7).


The biophysical properties and behavior of natural calf thymus DNA in hydrated 1-ethyl-3-butylimidazolium bromide ionic liquid ([C2bim]Br) have been studied using spectroscopy technique. The effect of ionic liquid concentration and temperature towards the duplex B-DNA conformation were determined. The presence of ionic liquid causes higher duplex DNA stability with the DNA melting temperature of ∼56°C without any addition of buffer solutions. The electrostatic attraction between ionic liquid’s cation and DNA phosphates groups was found play a main role in stabilizing native DNA structure. Understanding of the biophysical properties of DNA in this ionic media could be used as a platform for future development of specific solvent for nucleic acid nanotechnology.

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Additional Metadata

Item Type: Conference or Workshop Item (Paper)
Divisions: Faculty of Science
DOI Number: https://doi.org/10.1063/1.4968137
Publisher: AIP Publishing
Keywords: DNA; Ionic liquids; Biophysical properties; 1-ethyl-3-butylimidazolium bromide
Depositing User: Nabilah Mustapa
Date Deposited: 27 Sep 2017 10:13
Last Modified: 27 Sep 2017 10:13
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1063/1.4968137
URI: http://psasir.upm.edu.my/id/eprint/57425
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