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Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa)


Bilung, Lesley Maurice and Radu, Son and Bahaman, Abdul Rani and Abdul Rahim, Raha and Napis, Suhaimi and Cheah, Yoke Kqueen and Murugaiah, Chandrika and Abdul Hadi, Yousr and Robin, Tunung and Nishibuchi, Mitsuaki (2005) Random amplified polymorphic DNA-PCR typing of Vibrio parahaemolyticus isolated from local cockles (Anadara granosa). American Journal of Immunology, 1 (1). pp. 31-36. ISSN 1553-619X; ESSN: 1558-3775


Randomly amplified polymorphic DNA (RAPD) was used in this study to examine the genetic relatedness among the Vibrio parahaemolyticus strains. In the analysis by RAPD-PCR, the size for RAPD fragments ranged from 0.25 to 10.0 kb with average number of ten bands. The RAPD profiles revealed a high level of DNA sequence diversity within the Vibrio parahaemolyticus strains tested. Hence, this study, demonstrated that the local cockles (Anadara granosa) in the study area are populated by genetically polymorphic strains of V. parahaemolyticus. In addition, RAPD-PCR is simple, robust and sensitive typing methods to differentiate the V. parahaemolyticus strains.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Biotechnology and Biomolecular Sciences
Faculty of Food Science and Technology
Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.3844/ajisp.2005.31.36
Publisher: Science Publications
Keywords: RAPD-PCR; Cockles; Vibrio parahaemolyticus
Depositing User: Nabilah Mustapa
Date Deposited: 07 Sep 2017 03:47
Last Modified: 07 Sep 2017 03:47
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.3844/ajisp.2005.31.36
URI: http://psasir.upm.edu.my/id/eprint/57076
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