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Purification of recombinant nucleocapsid protein of Newcastle disease virus from unclarified feedstock using expanded bed adsorption chromatography


Citation

Tan, Yan Peng and Tau, Chuan Ling and Tan, Wen Siang and Yusoff, Khatijah and Tey, Beng Ti (2006) Purification of recombinant nucleocapsid protein of Newcastle disease virus from unclarified feedstock using expanded bed adsorption chromatography. Protein Expression and Purification, 46 (1). pp. 114-121. ISSN 1046-5928

Abstract / Synopsis

In the present work, a single-step purification of recombinant nucleocapsid protein (NP) of the Newcastle disease virus (NDV) directly from unclarified feedstock using an expanded bed adsorption chromatography (EBAC) was developed. Streamline 25 column (ID = 25 mm) was used as a contactor and Streamline chelating adsorbent immobilized with Ni2+ ion was used as affinity adsorbent. The dynamic binding capacity of Ni2+-loaded Streamline chelating adsorbent for the NP protein in unclarified feedstock was found to be 2.94 mg ml−1 adsorbent at a superficial velocity of 200 cm h−1. The direct purification of NP protein from unclarified feedstock using expanded bed adsorption has resulted in a 31% adsorption and 9.6% recovery of NP protein. The purity of the NP protein recovered was about 70% and the volume of processing fluid was reduced by a factor of 10. The results of the present study show that the IMA-EBAC developed could be used to combine the clarification, concentration and initial purification steps into a single-step operation.


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Additional Metadata

Item Type: Article
Divisions: Faculty of Engineering
Publisher: Elsevier
Keywords: NP protein, NDV, IMA-EBAC, Escherichia coli, Dynamic binding capacity
Depositing User: Erni Suraya Abdul Aziz
Date Deposited: 16 Apr 2010 08:45
Last Modified: 21 Jan 2016 01:56
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1016/j.pep.2005.06.015
URI: http://psasir.upm.edu.my/id/eprint/5607
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