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Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft


Ismail, Ruzila and Allaudin, Zeenathul Nazariah and Abdullah, Rasedee and Mohd Lila, Mohd Azmi and Nik Abd Rahman, Nik Mohd Afizan and Abdul Rahman, Sheikh Omar (2016) Combination of VP3 and CD147-knockdown enhance apoptosis and tumor growth delay index in colorectal tumor allograft. BMC Cancer, 16. art. no. 461. pp. 1-13. ISSN 1471-2407


Background: Cancer therapies that kill cancer cells without affecting normal cells is the ultimate mode of treating cancers. The VP3, an avian virus-derived protein, can specifically initiate cell death through several signal transduction pathways leading to apoptosis. In cancer, chemoresistance and cell survivability implicate the cell surface protein, CD147. Methods: In this study, transfection of VP3 and silencing of CD147 genes was achieved through the treatment of tumors with pVIVO1-GFP/VP3 (VP3), psiRNA-CD147/2 (shCD147/2), and their combination of CT26 colon cancer cell-induced in mice. The effectiveness of tumor-treatment was ascertained by electrophoresis, TUNEL assay, and flow cytometry analysis. While histopathological and biochemical analysis were used as toxic side effect identification. Results: The tumor growth delay index (TGDI) after treatment with VP3, shCD147/2, and their combination treatments increased by 1.3-, 1.2-, 2.0- and 2.3-fold respectively, over untreated control. The VP3-shCD147/2 combination treatment was more efficacious then either VP3 or shCD147/2 alone in the retardation of mouse CT26 colorectal cell tumor allograft. Conclusion: The antitumor effect of the combination treatment is the result of synergistic effects of VP3 and shCD147/2 on the tumor cells resulting in apoptosis. Thus, the study shows that combination of VP3 and shCD147/2 treatment can be developed into a potential approach for anticolorectal cancer treatment regimen.

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Additional Metadata

Item Type: Article
Divisions: Faculty of Veterinary Medicine
DOI Number: https://doi.org/10.1186/s12885-016-2530-8
Publisher: BioMed Central
Keywords: PVIVO1-GFP/VP3; PsiRNA-CD147/2; CT26 colon cancer cell tumor; Apoptosis
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 07 Nov 2017 02:31
Last Modified: 30 Oct 2019 07:27
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/s12885-016-2530-8
URI: http://psasir.upm.edu.my/id/eprint/55310
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