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Clausenidin induces caspase-dependent apoptosis in colon cancer


Waziri, Peter M. and Abdullah, Rasedee and Swee, Keong Yeap and Omar, Abdul Rahman and Kassim, Nur Kartinee and Malami, Ibrahim and Chee, Wun How and Christopher Etti, Imaobong and Abu, Mary Ladidi (2016) Clausenidin induces caspase-dependent apoptosis in colon cancer. BMC Complementary and Alternative Medicine, 16 (256). pp. 1-13. ISSN 1472-6882


Background: Clausena excavata burm.f. Is a shrub traditionally used to treat cancer patients in asia. The main bioactive chemical components of the plant are alkaloids and coumarins. In this study, we isolated clausenidin from the roots of c. Excavata to determine its apoptotic effect on the colon cancer (ht-29) cell line. Method: We examined the effect of clausenidin on cell viability, ros generation, dna fragmentation, mitochondrial membrane potential in ht-29 cells. Ultrastructural analysis was conducted for morphological evidence of apoptosis in the treated ht-29 cells. In addition, we also evaluated the effect of clausenidin treatment on the expression of caspase 3 and 9 genes and proteins in ht-29 cells. Result: Clausenidin induced a g0/g1 cell cycle arrest in ht-29 cells with significant (p < 0.05) dose-dependent increase in apoptotic cell population. The dna fragmentation assay also showed apoptotic features in the clausenidin-treated ht-29 cells. Clausenidin treatment had caused significant (p < 0.05) increases in the expression of caspase 9 protein and gene in ht-29 cells and mitochondrial ros and mitochondrial membrane depolarization. The results suggest the involvement of the mitochondria in the caspase-dependent apoptosis in clausenidin-treated colon cancer cells. Conclusion: Clausenidin induces a caspase-dependent apoptosis in colon cancers through the stimulation of the mitochondria. The study demonstrates the potential of clausenidin for use in the treatment of colon cancers.

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Additional Metadata

Item Type: Article
Divisions: Institute of Bioscience
DOI Number: https://doi.org/10.1186/s12906-016-1247-1
Publisher: BioMed Central
Keywords: Clausenidin; Apoptosis; Colon cancer; MMP; Caspase 9; Bcl 2; Bax
Depositing User: Mohd Hafiz Che Mahasan
Date Deposited: 07 Nov 2017 02:48
Last Modified: 07 Nov 2017 02:48
Altmetrics: http://www.altmetric.com/details.php?domain=psasir.upm.edu.my&doi=10.1186/s12906-016-1247-1
URI: http://psasir.upm.edu.my/id/eprint/55288
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